Staff Research Associate I - 135507

The UCSD Department of Pharmacology is one of two basic science units in the School of Medicine where world-class researchers investigate the effects of chemical interventions on biological systems. The faculty are internationally renowned for their strength in elucidating basic cellular and molecular mechanisms of signal transduction that lead to innovative drug target identification and ultimately to cutting edge drug discovery. The faculty also plays a major role in medical and graduate student training, providing educational and programmatic offerings that span several disciplines, and provide diversity to meet the interests of a broad spectrum of students and scholars. More than one hundred trainees at the graduate student and postdoctoral level, as well as a staff of more than 50 research technicians and department administrators interact closely with the faculty. The diverse mix of ages, backgrounds, and talents creates a robust work environment with challenging career opportunities and a commitment to continued growth potential. We constantly seek to recruit highly motivated, technologically-advanced and interested individuals to become a part of our dynamic exploratory research and educational environment. For more information, please visit our website at http://pharmacology.ucsd.edu.

Under supervision, incumbents assist in performing standard repetitive, technical laboratory and experimental procedures and receive training in the more difficult procedures related to blood-brain barrier research. Provide mouse colony maintenance for the Principal Investigator and other lab members. Under close technical supervision, perform procedures including endothelial cell and pericyte cell purification, cell culture, RNA purification, DNA purification, assist with qRT-PCR, RNA sequencing analysis. Responsible for laboratory management to include inventory, ordering supplies, maintaining equipment and ensure that EH&S regulations are met. Participate in data analysis and presentations. Incumbent is required to have theoretical knowledge in mammalian molecular and cellular biology.

• Theoretical knowledge of cell/molecular biology, biochemistry and/or other demonstrated equivalent combination of education and experience.

• Husbandry experience and mouse colony maintenance.

• Knowledge of general laboratory techniques, and scientific calculations sufficient to prepare and maintain complex chemical stocks such as dilutions, buffers, reagents and solutions.

• Knowledge/experience of molecular, biological techniques such as electrophoresis, blotting, PCR, cell biology, cell purification and basic mammalian cell culture techniques.

• Experience working with routine isolation of bacterial plasmids. Culture live tissue using aseptic techniques, basic imaging.

• Experience of blood brain barrier biology. Knowledge of endothelial cell and pericyte cells.

• Experience in handling small animals, preferably both rats and mice.

• Demonstrated ability to properly use and maintain laboratory equipment including centrifuges, microscopes, scales and computers.

• Organizational skills and ability to handle laboratory budgets and financial accountability, safety, security, inventory, equipment maintenance and storage.

• Ability to accurately maintain detailed computerized records and hard copy files of experimental records, inventory, and facility usage mouse genealogy, etc.

• Sufficient experience to assist senior researchers with biotechnological techniques related to microscopy; immunohistological techniques and physiochemical techniques, molecular cloning, immunofluorescent staining.

• Demonstrated ability to communicate with colleagues and superiors, including asking questions and notifications of problems.

• Excellent interpersonal skills to communicate in person, on the phone, and in writing to a diverse population including persons from foreign countries, the business community, academicians, students and staff with a high degree of diplomacy, mature judgment, tact and flexibility.

• Experience using computerized office software such as Internet Explorer, PowerPoint, Word, Excel, Adobe Photoshop, Reader, Illustrator, and Acrobat and provide assistance as needed.

• Knowledge of MSDS's (Material Safety Data Sheets), and the hazard communication and Injury and Illness Prevention Program. Knowledge of laboratory safety precautions and proper handling of chemicals & biohazardous materials, including appropriate clothing and shoes and use of other protective items.

• Ability to provide work direction to students in dish washing and making stock solution.

• Skill in working in a fast paced, research environment with frequent interruptions while completing projects and work assignments in a timely and efficient manner. Ability to organize, prioritize, and meet deadlines.

• Experience with qRT-PCR, RNA sequencing analysis.

• BS/BA, or equivalent, in biological sciences, chemistry, and microscopy optics or related field.

• Employment is subject to a criminal background check.

• Willingness to work evenings and weekends, if necessary.

• Willingness to work with animals, mice, rodents rats.

• Ability to work safely with biohazards materials.