Research Technician I

Massachusetts General Hospital
United States, Massachusetts, Boston
149 13th Street (Show on map)
Jan 07, 2025
The Research Technician I will perform standard laboratory procedures in the laboratory of Dr Marneros at the Cutaneous Biology Research Center/MGH. Duties include: Mouse colony management and husbandry including Tag and tail, genotyping, and euthanasia and tissue harvest, as well as phenotypic characterization of mouse phenotypes. Other responsibilities will include routine molecular biology, histology/immunochemistry, microscopy and flow cytometry in support of projects in cutaneous biology and immunology. Our laboratory is investigating molecular mechanisms involved in wound healing, angiogenesis and inflammation. Our laboratory is investigating molecular mechanisms involved in wound healing, angiogenesis and inflammation. Project 1: We investigate the role of proangiogenic factors for wound healing and age-related diseases. We could show that the proangiogenic factor VEGF-A induces oxidative stress and NLRP3 inflammasome activation to promote age-related diseases (Cell Reports, 2013; FASEB J, 2014; EMBO Mol Med, 2016; FASEB J, 2018, eLife, 2020). We use a variety of in vivo and in vitro approaches to investigate a novel pathogenic link between VEGF-A and NLRP3 inflammasome activation. Project 2: We identified a novel pathway that is critical for epithelial differentiation, especially in the kidney and skin. This new pathway plays a critical role in kidney and skin development *(Dev Cell, 2020; Cell Reports, 2021; J Clin Invbest, 2024). We are exploring key novel molecular mechanisms that regulate skin and kidney development, with implications for several skin and kidney diseases. In summary, our laboratory uses a large number of diverse experimental approaches (human genetics, mouse genetics, chemical screens, in vitro* assays, cutting-edge imaging technology) to define novel mechanisms in angiogenesis, wound healing and inflammation. All of our projects have strong translational clinical relevance. Our laboratory is embedded in a highly productive and well-equipped environment at the Cutaneous Biology Research Center of Massachusetts General Hospital/Harvard Medical School. Cutting-edge technologies are available within our Department and the MGH/Harvard research community. lab website: https://www.massgeneral.org/dermatology/research/cutaneous-biology-research-center/faculty-labs/alexander-marneros-lab Interested candidates should send the CV and references to Alexander G. Marneros, M.D., Ph.D., Email: amarneros@mgh.harvard.edu Principal Duties and Responsibililtes Prepares basic solutions and performs base-level procedures as assigned (i.e. Pipetting, cell and tissue culture, molecular biology and biochemistry procedure etc). There will be routine maintenance work for supporting the laboratory such as general laboratory. Perform routine assays, including RT-PCR, western blots, tissue processing, stainings and immunolabeling Conduct analysis of results Will assist in procedures with rodent models Maintain a laboratory notebook Attend weekly laboratory meetings and maintains laboratory notebooks. Will perform other duties as assigned Skills/Abilites Must exhibit initiative and is able to work independently when trained. Must have technical ability, proficient, and accurate in scientific experimentation. Ability to work in a group environment and be of assistance to a number of research projects. Excellent written and verbal communication skills Education Bachelor of Science/Art required Experience Prior laboratory experience preferred, especially in working with mice. The General Hospital Corporation is an Equal Opportunity Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.